CNE1-CAS9 cell line

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CNE1-CAS9 (Hela-contaminated cells, not for sales) Order

CNE1-CAS9 cell line
Catalog#
YC-C062-Cas9-H
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
CNE1-CAS9 cell line
Order

Detailed Product Information

Product category
Cas9 Stable Cell Lines
cell name
CNE1-CAS9
Cell Morphology
Endothelial cells, adherent
Passage ratio
1:3~1:5
Species
Human
Expression gene
Cas9
Resistance gene
hygro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
Description
Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the CNE1 cell line, screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a CNE1-CAS9 cell line that can maintain long-term, stable and high expression.
Cutting Efficiency Validation
CNE1-CAS9 cell line Product Validation Data(RT-QPCR)
Note: The above figure shows the sequencing peaks of the CNE1-CAS9 stable cell pool which is electroporated by SETD2 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:CNE1
STR Info (Cell bank)
Cell Line:CNE-1
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 101213.3101213.3
D7S820 10121012
D16S539 910910
VWA 14161416
TH01 679.0679.0
AMEL XXXX
TPOX 812812
CSF1PO 10111011
D12S391 2021
FGA 1821
D2S1338 1723
D21S11 2730
D18S51 1316
D8S1179 1216
D3S1358 1518
D6S1043 1118
PENTAE 1720
D19S433 1313
PENTAD 912
D1S1656 121516.0
* Hela-contaminated cells, not for sales
Reviewed by ICLAC (ref: 121113, 150506). STR profiling in Chan et al (2008) demonstrated similarity to HeLa, but at less than 80% match. Strong et al (2014) presented additional data in support of this finding, demonstrating that cells carry HPV-18, as previously demonstrated in HeLa cells. The authors speculate that a fusion event may have occurred between HeLa and an unknown cell line; although not proven, current data are consistent with this hypothesis.

Product Advantage

  • Easy to use:Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
    01
  • High-efficient KO:These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
    02
  • High-quality cell:Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
    03
CNE1-CAS9 cell line
CNE1-CAS9
Catalog#
YC-C062-Cas9-H
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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